SCBN cells
Catalog #:
P0010001Shipping advice:
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for details
Frozen vial | USD450.0 |
Origin:
Normal, non-tumorigenicSpecies:
CanineTissue:
DuodenumMorphology:
EpithelialProperties:
AdherentMedium:
DMEM + 10% FBSSubculture:
1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree CFreezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Sterility:
Pathogens:
Related cells by Organ:
References for SCBN cells:
1. van der Merwe, J. Q., Moreau, F., and MacNaughton, W. K. Protease-activated receptor-2 stimulates intestinal SCBN epithelial chloride transport through activation of PLC and selective PKC isoforms. Am J Physiol Gastrointest Liver Physiol, 296: G1258-1266, 2009.
2. van der Merwe, J. Q., Ohland, C. L., Hirota, C. L., and MacNaughton, W. K. Prostaglandin E2 derived from cyclooxygenases 1 and 2 mediates intestinal SCBN epithelial ion transport stimulated by the activation of protease-activated receptor 2. J Pharmacol Exp Ther, 329: 747-752, 2009.
3. Dong, X., Smoll, E. J., Ko, K. H., Lee, J., Chow, J. Y., Kim, H. D., Insel, P. A., and Dong, H. P2Y receptors mediate Ca2+ signaling in duodenocytes and contribute to duodenal mucosal SCBN bicarbonate secretion. Am J Physiol Gastrointest Liver Physiol, 296: G424-432, 2009.
4. Buret, A. and Lin, Y. C. Genotypic characterization of an epithelial cell line SCBN for the study of parasite-epithelial interactions. J Parasitol, 94: 545-548, 2008.
5. Smith, A., Contreras, C., Ko, K. H., Chow, J., Dong, X., Tuo, B., Zhang, H. H., Chen, D. B., and Dong, H. Gender-specific protection of estrogen against gastric acid-induced duodenal injury: stimulation of duodenal mucosal SCBN bicarbonate secretion. Endocrinology, 149: 4554-4566, 2008.
6. van der Merwe, J. Q., Hollenberg, M. D., and MacNaughton, W. K. EGF receptor transactivation and MAP kinase mediate proteinase-activated receptor-2-induced chloride secretion in intestinal epithelial SCBN cells. Am J Physiol Gastrointest Liver Physiol, 294: G441-451, 2008.