SK-MEL-1 cells
Catalog #:
C0020005
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| Frozen vial | USD420.0 |
Disease:
Malignant melanoma
Origin:
This cell line was isolated using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.
Species:
Homo sapiens, human
Malignant melanoma; skin; derived from metastatic site: lymphatic system
Melanoma
Spherical, clusters in suspension, dark color pellet after centrifugation.
Male, Caucasian, 29 years of age.
AddexBio-formulated EMEM (C0005-01)
+ 10% FBS
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 10^5 cells/mL and maintain at 2 x 10^5 cells/mL. Allow the flask to sit at room temperature (or at 37°C). Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days depending on cell density.
Complete growth medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818:12,13
D7S820: 12
THO1:6
TPOX: 11
vWA: 16,17
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-MEL-1 (AddexBio C0020005)
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Citation Format:
References for SK-MEL-1 cells:
1. Oettgen HF, Aoki T, Old LJ, Boyse EA, de Harven E, Mills GM. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J Natl Cancer Inst. 1968;41(4):827-843. PMID: 4879578
