WIL2-S cells

Catalog #:

C0003018

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Disease:

Hereditary spherocytosis

Origin:

This is a HAT sensitive variant of the WIL2 human B cell line.

Species:

Homo sapiens

Tissue:

Spleen

Morphology:

Lymphoblast

Properties:

Suspension, singly or in clumps

Patient:

Male, Caucasian, 5 years of age

Medium:

RPMI-1640 Medium + 10% FBS

Subculture:

Maintain at 0.3-1.0 x 10^6 cells/ml; feed cells twice-weekly, split of ca. 1:3 to 1:5; seed out at ca. 1 x 10^5 cells/ml. Culture at 5% CO2; 37°C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO