Hep3B cells
Catalog #:
C0015001
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for detailsShipping advice:
| Frozen vial | USD420.0 |
 |
 |
Disease:
Hepatocellular carcinoma
Established from the tumor tissue of an 8-year-old black boy
Homo sapiens
Liver
Epithelial
Adherent
Human hypotriploid stemline with near tetraploid side line
Male, 8 years of age
AddexBio-formulated EMEM (C0005-01) + 10% FBS
1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
II
Sterility:
Pathogens:
Protein Expression:
Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins
DNA Profile (STR):
Amelogenin: X;
CSF1PO: 8;
D13S317: 12,14;
D16S539: 10;
D5S818: 13;
D7S820: 8,10;
THO1: 6,7;
TPOX: 9;
vWA: 17
Related cells by Organ:
References for Hep3B cells:
1. Wang, J., Wang, X. F., Zhang, L. G., Xie, S. Y., Li, Z. L., Li, Y. J., Li, H. H., and Jiao, F. Involvement of the mitochondrial pathway in p53-independent apoptosis induced by p28GANK knockdown in Hep3B cells. Cytogenet Genome Res, 125: 87-97, 2009.
2. Ji, L., Shen, K., Liu, J., Chen, Y., Liu, T., and Wang, Z. Intracellular glutathione regulates Andrographolide-induced cytotoxicity on hepatoma Hep3B cells. Redox Rep, 14: 176-184, 2009.
3. Yildirim, H. and Kockar, F. TGF-beta upregulates tumor-associated carbonic anhydrase IX gene expression in Hep3B cells. Cell Biol Int, 33: 1002-1007, 2009.
4. Jin, C. Y., Park, C., Kim, G. Y., Lee, S. J., Kim, W. J., and Choi, Y. H. Genistein enhances TRAIL-induced apoptosis through inhibition of p38 MAPK signaling in human hepatocellular carcinoma Hep3B cells. Chem Biol Interact, 180: 143-150, 2009.
5. Zimonjic, D. B., Zhou, X., Lee, J. S., Ullmannova-Benson, V., Tripathi, V., Thorgeirsson, S. S., and Popescu, N. C. Acquired genetic and functional alterations associated with transforming growth factor beta type I resistance in Hep3B human hepatocellular carcinoma cell line. J Cell Mol Med, 13: 3985-3992, 2009.
6. Seo, S. H., Shin, D. Y., Hong, S. H., Park, S. E., Choi, Y. H., and Hong, S. H. Euphorbiae humifusae induces apoptosis in Hep3B hepatocarcinoma cells via simultaneous activation of the death receptor-mediated and mitochondrial pathways. Mol Med Report, 2: 221-227, 2009.
7.
Hien Dang, Steven N Steinway, Wei Ding and Carl B Rountree.
Induction of tumor initiation is dependent on CD44s in c-Met+ hepatocellular carcinoma.
BMC Cancer. 2015 Mar 21;15(1):161. doi: 10.1186/s12885-015-1166-4. PMID: 25886575
Certificate of Analysis/MSDS is available upon request with lot number
