TM3 cells

Catalog #:

T0028002

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Disease:

Normal cells

Species:

Mus musculus, mouse

Tissue:

Testis

Morphology:

Epithelial, Leydig cell

Properties:

Adherent

Medium:

A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculture:

Subculture sub-confluent cultures (85%) at 1:20 to 1:200 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO