BT-474 cells
Catalog #:
C0006012
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
 |
Disease:
Ductal Carcinoma
Origin:
This cell line was isolated from a solid, invasive ductal carcinoma of the breast.
Species:
Homo sapiens
Mammary gland/breast/duct
Epithelial
Adherent, patchy
The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14;?)(q32,?), unknown, iso(13q), der(6)t(6;7)(q21;q21), der(11)t(11;?)(14;?), del(11)(p11), unknown, unknown, der(2)t(2;?)(p21;?).
Female, 60 yrs of age, Caucasian
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 20% FBS + 10 ug/mL human insulin
Subculture at 1:3; feed cells 2 to 3 times weekly. Culture at 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,X
CSF1PO: 10,11
D13S317: 11
D16S539: 9,11
D5S818: 11,13
D7S820: 9,12
THO1: 7
TPOX: 8
vWA: 15,16
If use of this culture results in a scientific publication, it should be cited in the publication as: BT-474 (AddexBio C0006012)
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
DNA Profile (STR):
Citation Format:
Related cells by Organ:
References for BT-474 cells:
1. Lasfargues EY, Coutinho WG, Redfield ES. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J Natl Cancer Inst. 1978 Oct;61(4):967-78. PMID: 212572
