HEL 299 cells
Catalog #:
T0016051
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| Frozen vial | USD420.0 |
Disease:
Normal
Origin:
Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 15 population doublings before the onset of senescence.
Species:
Homo sapiens, human
Embryo lung
Fibroblast
Adherent
normal human male; diploid; stable. 2n = 46
Black, male, fetus.
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818:11,13
D7S820: 8,11
THO1:7
TPOX: 8,12
vWA: 16
If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
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Citation Format:
References for HEL 299 cells:
1. Peterson WD Jr, Stulberg CS, Swanborg NK, Robinson AR. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc Soc Exp Biol Med. 1968 Jul;128(3):772-776. PMID: 5668122
