H1975 [NCI-H1975] cells

Catalog #:

C0016013

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Disease:

Adenocarcinoma; non-small cell lung cancer

Origin:

The line was established in July 1988. The tissue donor was a non-smoker.

Species:

Human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Patient:

Female

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS

Subculture:

Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Split at 1:3 to 1:6. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO