LS-174T cells
Catalog #:
C0009013
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Disease:
Colorectal adenocarcinoma
Origin:
Dukes' type B. A trypsinised variant of LS180. The cells have microvilli and intracytoplasmic vacuoles.
Homo sapiens
Colon
Epithelial
Adherent
Modal no. 45 (2n). The cells are tumorigenic in nude mice.
Female, Caucasian, 58 yrs of age
AddexBio-formulated EMEM (C0005-01) + 10% FBS
1:3 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: LS-174T (AddexBio C0009013)
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DNA Profile (STR):
Amelogenin: X ;
CSF1PO: 10, 13, 14;
D13S317: 10;
D16S539: 11, 13;
D5S818: 11, 15;
D7S820: 11;
THO1: 6, 7;
TPOX: 8, 9;
vWA: 15, 17
Citation Format:
Related cells by Organ:
References for LS-174T cells:
1. Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976.