NIH-3T3 cells
Catalog #:
P0011001Shipping advice:
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
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Origin:
Normal, derived from NIH/Swiss mice embryoSpecies:
Mus musculus, NIH/SwissTissue:
EmbryoMorphology:
FibroblastProperties:
AdherentMedium:
DMEM + 10% FBSSubculture:
1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree CFreezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: NegativeSterility:
Pathogens:
References for NIH-3T3 cells:
1. Yoshii, H., Kamiyama, H., Minematsu, K., Goto, K., Mizota, T., Oishi, K., Katunuma, N., Yamamoto, N., and Kubo, Y. Cathepsin L is required for ecotropic murine leukemia virus infection in NIH3T3 cells. Virology, 394: 227-234, 2009.
2. Hampson, L., He, X. T., Oliver, A. W., Hadfield, J. A., Kemp, T., Butler, J., McGown, A., Kitchener, H. C., and Hampson, I. N. Analogues of Y27632 increase gap junction communication and suppress the formation of transformed NIH3T3 colonies. Br J Cancer, 101: 829-839, 2009.
3. Chien, C. W., Ho, I. C., and Lee, T. C. Induction of neoplastic transformation by ectopic expression of human aldo-keto reductase 1C isoforms in NIH3T3 cells. Carcinogenesis, 30: 1813-1820, 2009.
4. Miyata, M., Rikitake, Y., Takahashi, M., Nagamatsu, Y., Yamauchi, Y., Ogita, H., Hirata, K., and Takai, Y. Regulation by afadin of cyclical activation and inactivation of Rap1, Rac1, and RhoA small G proteins at leading edges of moving NIH3T3 cells. J Biol Chem, 284: 24595-24609, 2009.
5. Luo, X. G., Xi, T., Guo, S., Liu, Z. P., Wang, N., Jiang, Y., and Zhang, T. C. Effects of SMYD3 overexpression on transformation, serum dependence, and apoptosis sensitivity in NIH3T3 cells. IUBMB Life, 61: 679-684, 2009.
6. Ohshima, M., Yamaguchi, Y., Kappert, K., Micke, P., and Otsuka, K. bFGF rescues imatinib/STI571-induced apoptosis of sis-NIH3T3 fibroblasts. Biochem Biophys Res Commun, 381: 165-170, 2009.
