H520 (NCI-H520) cells
Catalog #:
C0016010
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| Frozen vial | USD420.0 |
Disease:
Squamous cell carcinoma
Origin:
This cell line was derived from a sample of a lung mass taken from a patient with squamous cell carcinoma of the lung
Species:
Homo sapiens
Lung
Epithelial
Adherent
This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%.
Male
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 10
D13S317: 10,11
D16S539: 8,13
D5S818: 12,13
D7S820: 8,12
THO1: 10
TPOX: 8
vWA: 18,19
If use of this culture results in a scientific publication, it should be cited in the publication as: H520 (AddexBio C0016010)
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References for H520 cells:
1. Banks-Schlegel SP, Gazdar AF, Harris CC. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 1985 Mar;45(3):1187-1197. PMID: 2578876
