SKNBE2 (SK-N-BE(2)) cells
Catalog #:
C0016055
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| Frozen vial | USD420.0 |
Origin:
Neuroblastoma
Origin:
The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Species:
Homo sapiens, human
Brain; derived from metastatic site: bone marrow
Neuroblast, neuroblastoma
Mixed, adherent and suspension
Modal number = 44; one or more HSR bearing chromosomes
Male, 2 years
F12 Medium/AddexBio-formulated EMEM 1:1 mixture (C0005-01) + 10% FBS
Subculture sub-confluent cultures (85%) at 1:12 to 1:20 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 9,11
D5S818:12
D7S820: 9,10
THO1:6,7
TPOX: 8,11
vWA: 18
If use of this culture results in a scientific publication, it should be cited in the publication as: SKNBE2 (AddexBio C0016055)
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Citation Format:
References for SKNBE2 cells:
1. Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PMID: 62055
