MDCKII cells
Catalog #:
P0014001Shipping advice:
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for details
| Frozen vial | USD450.0 |
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Origin:
NormalSpecies:
CanisTissue:
KidneyMorphology:
EpithelialProperties:
AdherentCytogenic data:
Non-tumorigenicMedium:
EMEM (C0005-01) + 10% FBSSubculture:
1:2 to 1:4using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree CFreezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSOBiosafety Level:
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: NegativeSterility:
Pathogens:
References for MDCKII cells:
1. Bottcher, E., Freuer, C., Steinmetzer, T., Klenk, H. D., and Garten, W. MDCK cells that express proteases TMPRSS2 and HAT provide a cell system to propagate influenza viruses in the absence of trypsin and to study cleavage of HA and its inhibition. Vaccine, 27: 6324-6329, 2009.
2. Dos Santos, P. M., Freitas, F. P., Mendes, J., Tararthuch, A. L., and Fernandez, R. Differential regulation of H+-ATPases in MDCK-C11 cells by aldosterone and vasopressin. Can J Physiol Pharmacol, 87: 653-665, 2009.
3. Tveit, H., Akslen, L. K., Fagereng, G. L., Tranulis, M. A., and Prydz, K. A secretory Golgi bypass route to the apical surface domain of epithelial MDCK cells. Traffic, 10: 1685-1695, 2009.
4. Abrahamyan, A., Nagy, E., and Golovan, S. P. Human H1 promoter expressed short hairpin RNAs (shRNAs) suppress avian influenza virus replication in chicken CH-SAH and canine MDCK cells. Antiviral Res, 84: 159-167, 2009.
5. Munoz, B., Huerta, M., and Lopez-Bayghen, E. Cilostazol reduces proliferation through c-Myc down-regulation in MDCK cells. Eur J Pharmacol, 616: 22-30, 2009.
6. de Souza, J., Benet, L. Z., Huang, Y., and Storpirtis, S. Comparison of bidirectional lamivudine and zidovudine transport using MDCK, MDCK-MDR1, and Caco-2 cell monolayers. J Pharm Sci, 98: 4413-4419, 2009.
