Human Pancreatic Ductal Epithelial Cells(HPDEC)
Catalog #:
T0018001
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Origin:
Immortalized pancreatic ductal epithelial cells.
Homo sapiens
Pancreas
Epithelial
Adherent
Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic
Keratinocyte SFM + Supplements
1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO
II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: NegativeSpecies:
Tissue:
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References for HPDE cells:
1. Kwon JJ, Willy JA, Quirin KA, Wek RC, Korc M, Yin XM, Kota J. Novel role of miR-29a in pancreatic cancer autophagy and its therapeutic potential. Oncotarget. 2016;7(44):71635-71650. doi: 10.18632/oncotarget.11928. PMID:27626694
2. Jorand R, Biswas S, Wakefield DL, Tobin SJ, Golfetto O, Hilton K, Ko M, Ramos JW, Small AR, Chu P, Singh G, Jovanovic-Talisman T. Molecular signatures of mu opioid receptor and somatostatin receptor 2 in pancreatic cancer. Mol Biol Cell. 2016;27(22):3659-3672. PMID:27682590
3. Boretti, M. I. and Gooch, K. J. Induced cell clustering enhances islet beta cell formation from human cultures enriched for pancreatic ductal epithelial cells (HPDEC). Tissue Eng, 12: 939-948, 2006.
4. Gatto, C., Callegari, M., Folin, M., Conconi, M., Paolin, A., Di Falco, G., Bredariol, S., Spinazzi, R., Parnigotto, P. P., and Nussdorfer, G. G. Effects of cryopreservation and coculture with pancreatic ductal epithelial cells (HPDEC) on insulin secretion from human pancreatic islets. Int J Mol Med, 12: 851-854, 2003.
5. Tai, M. H., Olson, L. K., Madhukar, B. V., Linning, K. D., Van Camp, L., Tsao, M. S., and Trosko, J. E. Characterization of gap junctional intercellular communication in immortalized human pancreatic ductal epithelial cells (HPDEC) with stem cell characteristics. Pancreas, 26: e18-26, 2003.
6. Chambers, J. A. and Harris, A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells (HPDEC). J Cell Sci, 105 ( Pt 2): 417-422, 1993.