H226 (NCI-H226) cells
Catalog #:
C0016011
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Frozen vial | USD420.0 |
Disease:
Squamous cell carcinoma; mesothelioma
Origin:
This cell line was derived from metastatic site: pleural effusion in March of 1980 from a patient with squamous cell carcinoma of the lung
Species:
Homo sapiens
Lung, Pleural effusion
Epithelial
Adherent
modal number = 47; range = 32-88; del(p25)
Male
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 13,14
D16S539: 9,12
D5S818: 11,12
D7S820: 8,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17
If use of this culture results in a scientific publication, it should be cited in the publication as: H226 (AddexBio C0016011)
Tissue:
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References for H226 cells:
1. NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.