HEK-293T cells
Catalog #:
T0011002
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Origin:
Embryonic kidney
Homo sapiens
Kidney
Epithelial
Adherent
transformed with Adeno and SV-40 viral DNA sequences
DMEM + 2 mM Glutamine + 10% FBS
1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Complete culture medium supplemented with 5% (v/v) DMSO
II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
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References for HEK-293T cells:
1. Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999.