MC116 cells
Catalog #:
C0003021
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
Disease:
Undifferentiated lymphoma
Origin:
Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.
Species:
Homo sapiens
Ascites
Lymphoblast
Suspension
45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23)
Male
RPMI-1640 Medium + 20% FBS
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 11^5 viable cells/mL and maintain at or below 3 X 10^6 cells/mL. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9.3,10
TPOX: 8,10
vWA: 15,17
Tissue:
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DNA Profile (STR):
References for MC116 cells:
1. Magrath IT, Pizzo PA, Whang-Peng J, Douglass EC, Alabaster O, Gerber P, Freeman CB, Novikovs L. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J Natl Cancer Inst. 1980 Mar;64(3):465-476. PMID: 6243721
