BSC40 cells
Catalog #:
T0011003
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Frozen vial | USD420.0 |
Origin:
BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31 to 40°C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.
Species:
Cercopithecus aethiops, African Green Monkey
Kidney
Epithelia
Adherent
African Green Monkey
DMEM Medium + 10% FBS
When reaching 80%, subculture using 0.25% trypsin or trypsin/EDTA at 1:6 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: BSC40 (AddexBio T0011003)
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References for BSC40 cells:
1. Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):942-946. PMID: 4362640