360 MMP FRET Substrate VI

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Synonyms: Dnp-PLAYWAR

Functional Activity:

This substrate has a fluorescent Trp residue in subsite P'2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P'1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P'1 and P'3 is optimized for MMP-8, while Arg is placed in P'4 to enhance solubility. Abs/Em = 280/360 nm.

Technical Data:

M.Wt: 1042.1

Sequence (one-letter code): Dnp-PLAYWAR

Sequence (three-letter code): Dnp - Pro - Leu - Ala - Tyr - Trp - Ala - Arg - OH

Excitation (nm): 280

Emission (nm): 360

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for 360 MMP FRET Substrate VI :

1. Aschi, M. et al. J. Comput. Aided. Mol. Des. 16, 213 (2002).

2. Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).

Certificate of Analysis/MSDS is available upon request

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