MEF (CF-1)
Catalog #:
P0011016
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| Frozen vial | USD145.0 |
Disease:
Normal, used for Stem Cell Research
Origin:
The cell line was established from embryonic day 14 (E14) CF-1 mouse embryos.
Species:
Mus musculus, mouse
Embryo
Fibroblast
Adherent
Male and female mixed; 14 days gestation
AddexBio-Formulated DMEM (C0003-01)) + 15% FBS
Subcultivation ratio of 4X10^3 cells/cm2 is recommended. Trypsinize cells using 0.25% (w/v) Trypsin-0.53 mM EDTA. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days. Reagents for cryopreservation is complete growth medium supplemented with 40% (v/v) FBS and 10% (v/v) DMSO.
1
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: MEF (CF-1) (AddexBio P0011016)
Tissue:
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