T47D cells
Catalog #:
C0006001
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Disease:
Breast ductal carcinoma
Derived from metastatic pleural effusion
Homo sapiens
Breast
Epithelial
Adherent
This is a hypotriploid human cell line
Female, 54 yrs of age
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
If use of this culture results in a scientific publication, it should be cited in the publication as: T47D (AddexBio C0006001)
Origin:
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Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
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Freezing Medium:
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Protein Expression:
calcitonin,
androgen receptor,
estrogen receptor,
progesterone receptor,
glucocorticoid receptor,
prolactin,
Isoenzymes:
AK-1, 1;
ES-D, 2;
G6PD, B;
GLO-I, 1-2;
PGM1, 1;
PGM3, 1
DNA Profile (STR):
Citation Format:
Related cells by Organ:
References for P47D cells:
1. Afhuppe, W., Sommer, A., Muller, J., Schwede, W., Fuhrmann, U., and Moller, C. Global gene expression profiling of progesterone receptor modulators in T47D cells provides a new classification system. J Steroid Biochem Mol Biol, 113: 105-115, 2009.
2. Chen, Y., Huang, K., Chen, K. E., and Walker, A. M. Prolactin and estradiol utilize distinct mechanisms to increase serine-118 phosphorylation and decrease levels of estrogen receptor alpha in T47D breast cancer cells. Breast Cancer Res Treat, 120: 369-377.
3. Holley, A. K., Kiningham, K. K., Spitz, D. R., Edwards, D. P., Jenkins, J. T., and Moore, M. R. Progestin stimulation of manganese superoxide dismutase and invasive properties in T47D human breast cancer cells. J Steroid Biochem Mol Biol, 117: 23-30, 2009.
4. Hua, D., Chen, B., Bai, M., Yu, H., Wu, X., and Jin, W. PEA3 activates VEGF transcription in T47D and SKBR3 breast cancer cells. Acta Biochim Biophys Sin (Shanghai), 41: 63-68, 2009.
5. Sanada, N., Gotoh, Y., Shimazawa, R., Klinge, C. M., and Kizu, R. Repression of activated aryl hydrocarbon receptor-induced transcriptional activation by 5alpha-dihydrotestosterone in human prostate cancer LNCaP and human breast cancer T47D cells. J Pharmacol Sci, 109: 380-387, 2009.
6. Zhang, Y., Zhao, H., Asztalos, S., Chisamore, M., Sitabkhan, Y., and Tonetti, D. A. Estradiol-induced regression in T47D:A18/PKCalpha tumors requires the estrogen receptor and interaction with the extracellular matrix. Mol Cancer Res, 7: 498-510, 2009.
