FHs 74 Int cells
Catalog #:
P0021002
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD420.0 |
 |
Disease:
Normal
Origin:
This cell line was isolated from human normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene.
Species:
Homo sapiens, human
Small intestine
Epithelial
Adherent
Normal female; diploid
Female, 3-4 months gestation
AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin)
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1:7,9.3
TPOX: 8
vWA: 16, 18
If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
DNA Profile (STR):
Citation Format:
References for FHs 74 Int cells:
1. Owens RB, Smith HS, Nelson-Rees WA, Springer EL. Epithelial cell cultures from normal and cancerous human tissues. J Natl Cancer Inst. 1976;56(4):843-849. PMID: 176412
