K6H6/B5 cells
Catalog #:
C0033002
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| Frozen vial | USD450.0 |
Disease:
Nodular lymphoma
Origin:
Mouse x Human hybrid myeloma. Derived by a fusion of P3/NSI/1-Ag4-1 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Tested and found negative for ectromelia virus (mousepox).
Species:
Human (B cell lymphoma)/mouse (myeloma) hybrid
Heterohybridoma, hybridoma
Lymphoblast
Suspension
AddexBio-formulated RPMI-1640 Medium(C0004-01)
, or equivalent medium
+ 10% FBS
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Tissue:
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Citation Format:
References for K6H6/B5 cells:
1. Carroll WL, Thielemans K, Dilley J, Levy R. Mouse x human heterohybridomas as fusion partners with human B cell tumors. J Immunol Methods. 1986 May 1;89(1):61-72. PMID: 3084658
