T84 cells
Catalog #:
C0009008
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Disease:
Colorectal carcinoma
Derived from metastatic lung
Homo sapiens
Colon
Epithelial
Adherent
Tumorigenic; 2n = 46, modal no. 56
Male, 72 years of age
DMEM/F12 + 10% FBS
1:2 TO 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: T84 (AddexBio C0009008)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Protein Expression:
keratin, peptide hormone, neurotransmitter, carcinoembryonic antigen (CEA)
Isoenzymes:
G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 1-2; AK-1, 1; GLO-1, 1-2
DNA Profile (STR):
Amelogenin: X;
CSF1PO: 10;
D13S317: 9;
D16S539: 10,11;
D5S818: 12;
D7S820: 8,10;
THO1: 6,9;
TPOX: 8,11;
vWA: 17,18
Citation Format:
Related cells by Organ:
References for T84 cells:
1. Beltran, A. R., Ramirez, M. A., Carraro-Lacroix, L. R., Hiraki, Y., Reboucas, N. A., and Malnic, G. NHE1, NHE2, and NHE4 contribute to regulation of cell pH in T84 colon cancer cells. Pflugers Arch, 455: 799-810, 2008.
2. Broekaert, I. J., Nanthakumar, N. N., and Walker, W. A. Secreted probiotic factors ameliorate enteropathogenic infection in zinc-deficient human Caco-2 and T84 cell lines. Pediatr Res, 62: 139-144, 2007.
3. Cai, W., Olafsen, T., Zhang, X., Cao, Q., Gambhir, S. S., Williams, L. E., Wu, A. M., and Chen, X. PET imaging of colorectal cancer in xenograft-bearing mice by use of an 18F-labeled T84.66 anti-carcinoembryonic antigen diabody. J Nucl Med, 48: 304-310, 2007.
4. Jia, F., Shelton, T. D., and Lewis, M. R. Preparation, characterization, and biological evaluation of a streptavidin-chimeric t84.66 conjugate for antibody pretargeting. Cancer Biother Radiopharm, 22: 654-664, 2007.
5. Mallegol, J., Van Niel, G., Lebreton, C., Lepelletier, Y., Candalh, C., Dugave, C., Heath, J. K., Raposo, G., Cerf-Bensussan, N., and Heyman, M. T84-intestinal epithelial exosomes bear MHC class II/peptide complexes potentiating antigen presentation by dendritic cells. Gastroenterology, 132: 1866-1876, 2007.
6. Matsumura, T., Fujinaga, Y., Jin, Y., Kabumoto, Y., and Oguma, K. Human milk SIgA binds to botulinum type B 16S toxin and limits toxin adherence on T84 cells. Biochem Biophys Res Commun, 352: 867-872, 2007.
