SJSA-1 cells
Catalog #:
C0004004
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Disease:
Osteosarcoma; multipotential sarcoma
Derived from primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur
Homo sapiens
Bone
Fibroblast
Adherent
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene
Male, Black, 19 years of age
AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS
1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004)
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References for SJSA-1 cells:
1. Bransfield, K. L., Askham, J. M., Leek, J. P., Robinson, P. A., and Mighell, A. J. Phenotypic changes associated with DYNACTIN-2 (DCTN2) over expression characterise SJSA-1 osteosarcoma cells. Mol Carcinog, 45: 157-163, 2006.
2. Missirlis, D., Khant, H., and Tirrell, M. Mechanisms of peptide amphiphile internalization by SJSA-1 cells in vitro. Biochemistry, 48: 3304-3314, 2009.
3. Missirlis, D., Krogstad, D. V., and Tirrell, M. Internalization of p53(14-29) peptide amphiphiles and subsequent endosomal disruption results in SJSA-1 cell death. Mol Pharm, 7: 2173-2184.