COLO 205 cells
Catalog #:
C0009018
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| Frozen vial | USD420.0 |
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Disease:
Dukes' type D, colorectal adenocarcinoma
Origin:
This line was isolated in 1975 from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Species:
Homo sapiens
Colon; metastatic site: ascites
Epithelial; round and refractile cells in suspension. Some cuboidal cells in the monolayer. COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.
Mixed, adherent and suspension
Hypertriploid modal no 71-78
70 year old Caucasian, male
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture at 1:6; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 205 (AddexBio C0009018)
Tissue:
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References for COLO 205 cells:
1. Semple TU, Quinn LA, Woods LK, Moore GE. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 1978 May;38(5):1345-1355. PMID: 565251
