TRAMP C2 cells
Catalog #:
C0019006
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD450.0 |
Disease:
Adenocarcioma
Origin:
The TRAMP-C2 cell line was derived in 1996 from a heterogeneous 32 week primary tumor in the prostate of a PB-Tag C57BL/6 (TRAMP) mouse.
Species:
Mus musculus, transgenic mouse
Prostate
Epithelial
Adherent
C57BL/6, Male, adult
AddexBio-formulated DMEM (C0003-01) + 5% FBS + 5% Nu-Serum IV + 0.005 mg/mL bovine insulin + 10 nM dehydroisoandrosterone
Subculture sub-confluent cultures (70-80%) at 1:6 to1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. An inoculum of 6x10^3 to 8x10^3 viable cells/cm2 is recommended. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO II (Cells contain SV40 viral DNA sequences)
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: TRAMP C2 (AddexBio C0019006)
Tissue:
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Citation Format:
References for TRAMP C2 cells:
1. Greenberg NM, DeMayo FJ, Sheppard PC, Barrios R, Lebovitz R, Finegold M, Angelopoulou R, Dodd JG, Duckworth ML, Rosen JM, et al. The rat probasin gene promoter directs hormonally and developmentally regulated expression of a heterologous gene specifically to the prostate in transgenic mice. Mol Endocrinol. 1994 Feb;8(2):230-239. PMID: 8170479
