360 MMP FRET Substrate V

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Synonyms: Dnp-RPLALWRS

Functional Activity:

An excellent fluorogenic substrate for MMP-7 having Km and kcat values of 26 mM and 5.0 s^-1, respectively. (kcat/Km = 1.9 x 105 M^-1 s^-1). The enzyme catalyzed cleavage occurs at the Ala-Leu bond. Abs/Em = 280/360 nm.

Technical Data:

M.Wt: 1164.3

Sequence (one-letter code): Dnp-RPLALWRS

Sequence (three-letter code): Dnp - Arg - Pro - Leu - Ala - Leu - Trp - Arg - Ser - OH

Excitation (nm): 280

Emission (nm): 360

Solubility: Soluble in DMSO

Purity: >95% (HPLC)

Shipping Conditions: Room temperature

Storage: Store and desiccate at -20°C and protect from light.

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References for 360 MMP FRET Substrate V :

1. Kraft, PJ. et al. Connect Tissue Res. 42, 149 (2001);

2. Itoh, M. et al. J. Pharm. Biomed. Anal. 15, 1417 (1997);

3. Welch, AR. et al. Arch. Biochem. Biophys. 324, 59 (1995).

Certificate of Analysis/MSDS is available upon request

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