BeWo Clone b30 cells
Catalog #:
C0030002
Shipping advice:
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details
| Frozen vial | USD470.0 |
Disease:
Choriocarcinoma
Origin:
This cell line was derived from a malignant gestational choriocarcinoma of the fetal placenta. This clone has proven useful in transport studies because the cells form polarized, confluent monolayers.
Species:
Homo sapiens
Placenta
Epithelial
Adherent
Modal number = 86; range = 71 to 178.
Stemline number is hypotetraploid. Karyotype relatively stable within stemline number.
Male
AddexBio-Optimized DMEM (C0003-02) + 10% FBS
Subculture at 1:4; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16
Tissue:
Morphology:
Properties:
Cytogenic data:
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Subculture:
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DNA Profile (STR):
References for BeWo subclone b30 cells:
1. Mitra P and Audus KL (2008) In Vitro Models and Multidrug Resistance Mechanisms of the Placental Barrier. In Vol VII Drug Absorption Studies, In Situ, In Vitro and In Silico Models (pp. 368-396)
2. Dimopoulou M, Verhoef A, Gomes CA, van Dongen CW, Rietjens IMCM, Piersma AH, van Ravenzwaay B. A comparison of the embryonic stem cell test and whole embryo culture assay combined with the BeWo placental passage model for predicting the embryotoxicity of azoles. Toxicol Lett. 2018 Apr;286:10-21. doi: 10.1016/j.toxlet.2018.01.009. PMID: 29337257
