A427 cells
Catalog #:
C0016004
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Disease:
Lung carcinoma
Established from the lung carcinoma of a 52-year-old Caucasian man
Homo sapiens
Lung
Epithelial
Adherent
hypotriploid to hypertriploid with abnormalities
Male, Caucasian, 52 yrs of age
DMEM/F12 + 10% FBS
1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: A427 (AddexBio C0016004)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Isoenzymes:
AK-1, 2;
ES-D, 1;
G6PD, B;
GLO-I, 1;
PGM1, 1-2;
PGM3, 1
DNA Profile (STR):
Amelogenin: X,Y;
CSF1PO: 10,12;
D13S317: 11,12;
D16S539: 11,13;
D5S818: 12;
D7S820: 8,12;
THO1: 9;
TPOX: 8,11;
vWA: 17
Related cells by Organ:
References for A427 cells:
1. Lu, X. and Arthur, G. The differential susceptibility of A427 and A549 cell lines to the growth-inhibitory effects of ET-18-OCH3 does not correlate with the relative effects of the alkyl-lysophospholipid on the incorporation of fatty acids into cellular phospholipids. Cancer Res, 52: 2813-2817, 1992.