B103 cells
Catalog #:
C0005003
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Rattus norvegicus (rat)
Brain
Epithelial
Adherent
AddexBio-formulated DMEM (C0003-01) + 10% FBS
1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: B103 (AddexBio C0005003)
Disease:
Neuroblastoma
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References for B103 cells:
1. Hahn, C. G., Pawlyk, A. C., Whybrow, P. C., and Tejani-Butt, S. M. Differential expression of thyroid hormone receptor isoforms by thyroid hormone and lithium in rat GH3 and B103 cells. Biol Psychiatry, 45: 1004-1012, 1999.
2. Han, J. Z., Lin, W., Lou, S. J., Qiu, J., and Chen, Y. Z. A rapid, nongenomic action of glucocorticoids in rat B103 neuroblastoma cells. Biochim Biophys Acta, 1591: 21-27, 2002.
3. Hashimoto, M., Takenouchi, T., Rockenstein, E., and Masliah, E. Alpha-synuclein up-regulates expression of caveolin-1 and down-regulates extracellular signal-regulated kinase activity in B103 neuroblastoma cells: role in the pathogenesis of Parkinson's disease. J Neurochem, 85: 1468-1479, 2003.
4. Kawanabe, Y., Hashimoto, N., and Masaki, T. B103 neuroblastoma cells predominantly express endothelin ET(B) receptor; effects of extracellular Ca(2+) influx on endothelin-1-induced mitogenesis. Eur J Pharmacol, 425: 173-179, 2001.
5. Pinto, C. S. and Seifert, R. Decreased GTP-stimulated adenylyl cyclase activity in HPRT-deficient human and mouse fibroblast and rat B103 neuroblastoma cell membranes. J Neurochem, 96: 454-459, 2006.
6.
Tagalakis AD, Kenny GD, Bienemann AS, McCarthy D, Munye MM, Taylor H, Wyatt MJ, Lythgoe MF, White EA, Hart SL. PEGylation improves the receptor-mediated transfection efficiency of peptide-targeted, self-assembling, anionicnanocomplexes. J Control Release. 2014 Jan 28;174:177-87. doi: 10.1016/j.jconrel.2013.11.014. PMID: 24269968
Certificate of Analysis/MSDS is available upon request with lot number