U-251MG cells
Catalog #:
C0005029
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| Frozen vial | USD450.0 |
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Disease:
Human glioblastoma astrocytoma
Origin:
Derived from a malignant glioblastoma tumor by explant technique.
Species:
Homo sapiens, human
Brain
Pleomorphic/astrocytoid
Adherent
2n = 46
AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10,000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818:11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18
If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Tissue:
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Citation Format:
References for U-251MG cells:
1. Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand. 1968;74(4):465-486. PMID: 4313504
