bEnd.3 (BEND3) cells
Catalog #:
C0005030
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Disease:
Endothelioma
Origin:
The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
Species:
Mus musculus, mouse
Brain, cerebral cortex
Endothelial, endothelialpolyoma middle T antigen transformed
Adherent
BALB/c, 6 weeks
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:6 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO II
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: bEnd.3 (AddexBio C0005030)
Tissue:
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Citation Format:
References for bEnd.3 cells:
1. Williams RL, Courtneidge SA, Wagner EF. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell. 1988 Jan 15;52(1):121-131. PMID: 3345558