C3H/10T1/2 Clone 8 cells
Catalog #:
P0011003
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| Frozen vial | USD450.0 |
Disease:
Sarcoma
Origin:
C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.
Species:
Mouse (Mus musculus) C3H strain
Embryo
Fibroblast
Adherent
Mouse karyotype with a modal number of 80 chromosomes.
MEM Medium + 10% FBS
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C.
Complete culture medium supplemented with 5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Tissue:
Morphology:
Properties:
Cytogenic data:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
References for C3H/10T1/2 Clone 8 cells:
1. Reznikoff CA, Bertram JS, Brankow DW, Heidelberger C. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 1973 Dec;33(12):3239-3249. PMID: 4796800
