INS-1 cells
Catalog #:
C0018007
Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for detailsShipping advice:
| Frozen vial | USD570.0 |
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Disease:
Insulinoma (Radiation induced cell immortalization)
Pancreatic Islets
Rattus Norvegicus
Pancreas
β cells
Adherent
AddexBio-Optimized RPMI-1640 Medium(C0004-02) + 10% FBS + 0.05 mM 2-mercaptoethanol
1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Respond to glucose within the physiological range. Relatively high insulin content.Origin:
Species:
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References for INS-1 cells:
1. Asfari, M., Janjic, D., Meda, P. et al. (1992). Establishment of 2-mercaptoethanol-dependent differentiated insulin- secreting cell lines. Endocrinology 130, 167-178.
2. Chang TJ, Tseng HC, Liu MW, Chang YC, Hsieh ML, Chuang LM. Glucagon-like peptide-1 prevents methylglyoxal-induced apoptosis of beta cells through improvingmitochondrial function and suppressing prolonged AMPK activation.Sci Rep. 2016 Mar 21;6:23403. doi: 10.1038/srep23403. PMID: 26997114
3. Fortin JS, Benoit-Biancamano MO. Inhibition of islet amyloid polypeptide aggregation and associated cytotoxicity by nonsteroidal anti-inflammatory drugs. Can J Physiol Pharmacol. 2016 Jan;94(1):35-48. PMID: 26524404
4. Fortin JS, Benoit-Biancamano MO. In vitro evaluation of hypoglycemic agents to target human islet amyloid polypeptide: a key protein involved in amyloid deposition and beta-cell loss. Can J Diabetes. 2015 Oct;39(5):373-382. PMID: 25862064
5. Bronsart LL, Stokes C, Contag CH.Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass.PLoS One. 2016 Jan 11;11(1):e0146601. PMID: 26752052
6. Fortin JS, Benoit-Biancamano MO. Wildlife sequences of islet amyloid polypeptide (IAPP) identify critical species variants for fibrillization. Amyloid. 2015;22(3):194-202. PMID: 26300107
7. Lee SJC, Choi TS, Lee JW, Lee HJ, Mun D, Akashi S, Lee SW, Lim MH, Kim HI. Structure and assembly mechanisms of toxic human islet amyloid polypeptide oligomers associated with copper. Chem Sci. 2016;22:5398-5406.
8. Lombardi A, Trimarco B, Iaccarino G, Santulli G. Impaired mitochondrial calcium uptake caused by tacrolimus underlies beta-cell failure. Cell Commun Signal. 2017;15(1):47. doi: 10.1186/s12964-017-0203-0. PMID: 29132395
9. Wong JC, Vo V, Gorjala P, Fiscus RR. Pancreatic-beta-cell survival and proliferation are promoted by protein kinase G type I-alpha and downstream regulation of AKT/FOXO1. Diab Vasc Dis Res. 2017;14(5):434-449. doi: 10.1177/1479164117713947. PMID: 28631500
10. Ji J, Petropavlovskaia M, Khatchadourian A, Patapas J, Makhlin J, Rosenberg L, Maysinger D. Type 2 diabetes is associated with suppression of autophagy and lipid accumulation in ??cells. J Cell Mol Med 2019 Feb 1. doi: 10.1111/jcmm.14172. PMID: 30710421
