K562 cells
Catalog #:
C0003004
Use only Express Delivery (overnight) for cell lines. International shipping rate please contact us for detailsShipping advice:
| Frozen vial | USD420.0 |
 |
Disease:
Chronic myelogenous leukemia (CML)
Established from pleural effusion
Homo sapiens
Blood
Lymphoblast
Suspension
The stemline chromosome number is triploid
Female, 53 yrs of age
IMDM +10% FBS
Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37°C.
Add fresh medium every 2 to 3 days.
Complete culture medium supplemented with 5% (v/v) DMSO
I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Medium Renewal:
Freezing Medium:
Biosafety Level:
Sterility:
Pathogens:
Isoenzymes:
AK-1, 1;
ES-D, 1;
G6PD, B;
GLO-I, 2;
Me-2, 0;
PGM1, 0;
PGM3, 1
DNA Profile (STR):
Amelogenin: X;
CSF1PO: 9,10;
D13S317: 8;
D16S539: 11,12;
D5S818: 11,12;
D7S820: 9,11;
THO1: 9.3;
TPOX: 8,9;
vWA: 16
Related cells by Organ:
CCRF-CEM
References for K562 cells:
1. Chen, Z., Luo, H. Y., Steinberg, M. H., and Chui, D. H. BCL11A represses HBG transcription in K562 cells. Blood Cells Mol Dis, 42: 144-149, 2009.
2. Konishi, E., Tabuchi, Y., and Yamanaka, A. A simple assay system for infection-enhancing and -neutralizing antibodies to dengue type 2 virus using layers of semi-adherent K562 cells. J Virol Methods, 163: 360-367.
3. Navarro, F., Gutman, D., Meire, E., Caceres, M., Rigoutsos, I., Bentwich, Z., and Lieberman, J. miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Blood, 114: 2181-2192, 2009.
4. Reuter, S., Charlet, J., Juncker, T., Teiten, M. H., Dicato, M., and Diederich, M. Effect of curcumin on nuclear factor kappaB signaling pathways in human chronic myelogenous K562 leukemia cells. Ann N Y Acad Sci, 1171: 436-447, 2009.
5. Salvatori, F., Cantale, V., Breveglieri, G., Zuccato, C., Finotti, A., Bianchi, N., Borgatti, M., Feriotto, G., Destro, F., Canella, A., Breda, L., Rivella, S., and Gambari, R. Development of K562 cell clones expressing beta-globin mRNA carrying the beta039 thalassaemia mutation for the screening of correctors of stop-codon mutations. Biotechnol Appl Biochem, 54: 41-52, 2009.
6. Wang, Y., Su, M., Yin, J., and Zhang, H. [Effect of nobiletin on K562 cells xenograft in nude mice]. Zhongguo Zhong Yao Za Zhi, 34: 1410-1414, 2009.
