HT-29 cells
Catalog #:
C0009004
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Disease:
Colorectal adenocarcinoma
Homo sapiens
Colon
Epithelial
Adherent
Tumorigenic, The stemline chromosome number is hypertriploid
Female, Caucasian, 44 yrs of age
McCoy's 5a (Gibco 16600082) + 10% FBS
1:3 to 1:8, (80-90%), 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Complete culture medium supplemented with 5% (v/v) DMSO
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: HT-29 (AddexBio C0009004)
Origin:
Species:
Tissue:
Morphology:
Properties:
Cytogenic data:
Patient:
Medium:
Subculture:
Freezing Medium:
Biosafety Level:
I
Sterility:
Pathogens:
Protein Expression:
HLA A1, A3, B12, B17, Cw5, CD4 -, cell surface expression of galactose ceramide, Urokinase receptor(u-PAR), vitamin D (moderate expression)
Isoenzymes:
Me-2, 1; PGM3, 1-2; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, BDNA Profile (STR):
Amelogenin: X,X;
CSF1PO: 11,12;
D13S317: 11,11;
D16S539: 11,12;
D5S818: 11,12;
D7S820: 10;
THO1: 6,9;
TPOX: 8,9;
vWA: 17,19;
D3S1358: 15,17;
D21S11: 29,30;
D18S51: 13;
Penta E: 14,16;
Penta D: 11,13;
D8S1179: 10;
FGA: 20,22
Citation Format:
Related cells by Organ:
References for HT-29 cells:
1. Brandenburg, L. O., Seyferth, S., Wruck, C. J., Koch, T., Rosenstiel, P., Lucius, R., and Pufe, T. Involvement of Phospholipase D 1 and 2 in the subcellular localization and activity of formyl-peptide-receptors in the human colonic cell line HT29. Mol Membr Biol, 26: 371-383, 2009.
2. Chen, J. and Huang, X. F. Comment on "effects of adipocyte-secreted factors on cell cycle progression in HT29 cells" published by Eur J Nutr. Eur J Nutr, 48: 505, 2009.
3. Klenow, S. and Glei, M. New insight into the influence of carob extract and gallic acid on hemin induced modulation of HT29 cell growth parameters. Toxicol In Vitro, 23: 1055-1061, 2009.
4. Selvakumar, P., Lakshmikuttyamma, A., Das, U., Pati, H. N., Dimmock, J. R., and Sharma, R. K. NC2213: a novel methionine aminopeptidase 2 inhibitor in human colon cancer HT29 cells. Mol Cancer, 8: 65, 2009.
5. Singh, N., Shrivastav, A., and Sharma, R. K. Curcumin induces caspase and calpain-dependent apoptosis in HT29 human colon cancer cells. Mol Med Report, 2: 627-631, 2009.
6. Wu, P. P., Jin, Y. L., Shang, Y. F., Jin, Z., Wu, P., and Huang, P. L. Restoration of DLC1 gene inhibits proliferation and migration of human colon cancer HT29 cells. Ann Clin Lab Sci, 39: 263-269, 2009.
