Wnt TCF/LEF signaling (Wnt Assay)

The TCF/LEF Reporter assay is designed to monitor the activity of Wnt signal transduction pathways in cultured cells. The Wnt signaling pathway leads to the dephosphorylation, stabilization, and nuclear translocation of β-catenin. The stabilized β-catenin complexes with the TCF/LEF transcription factors will then bind to the TCF/LEF transcriptional response element (TRE) and trigger the transcription of Wnt-responsive genes. The TCF/LEF -responsive luciferase construct encodes the firefly luciferase reporter gene under the control of a promoter of the TCF/LEF transcriptional response element (TRE).