Plasma Protein Binding

Drug efficacy and disposition are influenced by binding of a drug to plasma proteins, primarily human serum albumin, or α1-acid glycoprotein. High drug-protein binding can both reduce the fraction of free drug available for target sites and prolong the duration of drug activity. AddexBio will use equilibrium dialysis , ultrafiltration using Thermo rapid equilibrium dialysis (RED) device and HTDialysis 96-well micro-equilibrium dialysis device. Multiple concentrations can be tested to obtain K_d estimates. We can also use in vivo samples to assess binding values.