Cystic Fibrosis Transmembrane Conductance Regulator: CFTR Channels (Epithelial Ion Transport) Assay

Structure of the CFTR protein

CFTR Assay

Cystic fibrosis transmembrane conductance regulator (CFTR) channels are involved in numerous epithelial cell functions, such as regulation of pHi and cell volume, mucus secretion and expansion, development and growth. CFTR channels have been well recognized as therapeutic targets for treating cystic fibrosis and other diseases. Utilizing digital cell imaging with novel fluorescent dye in epithelial cell lines overly expressing wild type CFTR or mutation F508δ-CFTR, we offer assays to test effects of lead compounds on CFTR activity in both epithelial cell lines and primary human epithelial cells.

CFTR Assay Price Structure:

US$80 for 1 concentration to 1 single cell.

CFTR Channel and Ion Transport

CFTR channels play an important role in epithelial ion transport, such as Cl^- and HCO^3- secretions, which are dysfunctional in cystic fibrosis. Utilizing Ussing chamber and pH stat measurement, we also offer assays to test effects of lead compounds on CFTR function in epithelia, such as Cl^- and HCO^3- secretions from primary human epithelial cells, small intestinal and colonic epithelia of animals.

Ion Transport Price Structure:

US$600 for 1 concentration to 1 chamber for each measurement.

Related references to CFTR

1. Berdiev BK, Qadri YJ, and Benos DJ. Assessment of the CFTR and ENaC association. Mol Biosyst 5: 123-127, 2009.
2. Bremer LA, Blackman SM, Vanscoy LL, McDougal KE, Bowers A, Naughton KM, Cutler DJ, and Cutting GR. Interaction between a novel TGFB1 haplotype and CFTR genotype is associated with improved lung function in cystic fibrosis. Hum Mol Genet 17: 2228-2237, 2008.
3. da Costa MZ, Guarita DR, Ono-Nita SK, Nogueira Jde A, Nita ME, Paranagua-Vezozzo DC, de Souza MT, do Carmo EP, Teixeira AC, and Carrilho FJ. CFTR polymorphisms in patients with alcoholic chronic pancreatitis. Pancreatology 9: 173-181, 2009.
4. Liu Y, Wang Y, Jiang Y, Zhu N, Liang H, Xu L, Feng X, Yang H, and Ma T. Mild processing defect of porcine DeltaF508-CFTR suggests that DeltaF508 pigs may not develop cystic fibrosis disease. Biochem Biophys Res Commun 373: 113-118, 2008.
5. Noel S, Strale PO, Dannhoffer L, Wilke M, DeJonge H, Rogier C, Mettey Y, and Becq F. Stimulation of salivary secretion in vivo by CFTR potentiators in Cftr+/+ and Cftr-/- mice. J Cyst Fibros 7: 128-133, 2008.
6. Paranjape SM and Zeitlin PL. Atypical cystic fibrosis and CFTR-related diseases. Clin Rev Allergy Immunol 35: 116-123, 2008.
7. Zheng W, Kuhlicke J, Jackel K, Eltzschig HK, Singh A, Sjoblom M, Riederer B, Weinhold C, Seidler U, Colgan SP, and Karhausen J. Hypoxia inducible factor-1 (HIF-1)-mediated repression of cystic fibrosis transmembrane conductance regulator (CFTR) in the intestinal epithelium. Faseb J 23: 204-213, 2009.