B16-F10 cells
Catalog #:
C0012005
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| Frozen vial | USD420.0 |
Disease:
Melanoma
Origin:
Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.
Species:
Mus musculus, mouse
Skin
Mixture of spindle-shaped and epithelial-like cells
Adherent
C57BL/6J
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.
Complete growth culture medium supplemented with 7.5% (v/v) DMSO I
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
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Citation Format:
References for B16-F10 cells:
1. Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 1975 Jan;35(1):218-224. PMID: 1109790
