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Intracellular pH Regulators (pHi Regulators)

Protein Structure of NHE

Importance of Intracellular pH regulator

Intracellular pH (pHi) is important for maintaining various cell functions. Under physiological conditions, pHi is fine controlled by several ion channels and exchangers, including Na+/H+ exchanger (NHE), Na+-HCO3- co-transporter (NBC), Cl-/HCO3- anion exchangers (AE) and CFTR channels. However, pHi is dysregulated in a number of cardiac and neural pathologies, and development of various cancers. Using digital cell imaging with fluorescent dye in single human living cells, we offer assays to test effects of lead compounds on several pHi regulators, including NHE, NBC, NE and CFTR channels in both cell lines and primary human cells.

Diagram of functions of Intracellular pH regulators

Price Structure of Intracellular pH Regulator Assay:

US$80 for 1 concentration to 1 single cell with 1 pHi regulator.

Related References to Intracellular pH Regulators:

  1. Amlal H, Burnham CE, and Soleimani M. Characterization of Na+/HCO-3 cotransporter isoform NBC-3. Am J Physiol 276: F903-913, 1999.
  2. Azarani A, Orlowski J, and Goltzman D. Parathyroid hormone and parathyroid hormone-related peptide activate the Na+/H+ exchanger NHE-1 isoform in osteoblastic cells (UMR-106) via a cAMP-dependent pathway. J Biol Chem 270: 23166-23172, 1995.
  3. LaPointe MS, Ye M, Bacallao R, and Batlle D. NHE-1 protein in vascular smooth muscle and lymphocytes from the spontaneously hypertensive rat. Hypertension 30: 880-885, 1997.
  4. Romero MF. The electrogenic Na+/HCO3- cotransporter, NBC. Jop 2: 182-191, 2001.
  5. Siczkowski M and Ng LL. Glucose-induced changes in activity and phosphorylation of the Na+/H+ exchanger, NHE-1, in vascular myocytes from Wistar-Kyoto and spontaneously hypertensive rats. Metabolism 45: 114-119, 1996.
  6. Silviani V, Gastaldi M, Planells R, Marteau C, Massacrier A, Cohen P, Cau P, and Gerolami A. NHE-3 isoform of the Na+/H+ exchanger in human gallbladder. Localization of specific mRNA by in situ hybridization. J Hepatol 26: 1281-1286, 1997.
  7. Soleimani M. Na+:HCO3- cotransporters (NBC): expression and regulation in the kidney. J Nephrol 15 Suppl 5: S32-40, 2002.
  8. Soleimani M and Burnham CE. Na+:HCO(3-) cotransporters (NBC): cloning and characterization. J Membr Biol 183: 71-84, 2001.

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