Disease:

Choriocarcinoma

Origin:

This cell line was derived from a malignant gestational choriocarcinoma of the fetal placenta.

Species:

Homo sapiens

Tissue:

Placenta

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Modal number = 86; range = 71 to 178. Stemline number is hypotetraploid. Karyotype relatively stable within stemline number.

Patient:

Male

Medium:

Ham's F-12 Medium + 10% FBS

Subculture:

Subculture at 1:4; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO