REH cells

Catalog #:

C0003031

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Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details

Disease:

B cell precursor leukemia

Origin:

established from the peripheral blood of a 15-year-old North African girl with acute lymphoblastic leukemia (ALL at first relapse) in 1973; carries t(12;21) leading to ETV6-RUNX1 (TEL-AML1) fusion gene.

Species:

Homo sapiens, human

Tissue:

Peripheral blood

Morphology:

Small, round, single cells or cell clusters in suspension

Properties:

Suspension

Patient:

Black, female, 15 years of age.

Medium:

AddexBio-formulated RPMI-1640 (C0004-01) + 10-20% FBS

Subculture:

Initially after thawing cells may be difficult to culture! maintain at high density (>2 x 10^6 cells/ml); cells grow slowly at this stage (with doubling time at 50-70 hours); minimum density at ca. 0.5 x 10^6 cells/ml; seed out at ca. 2 x 10^6 cells/ml with initially 20% FBS in a 24-well plate; after thawing viability decreases during the first several days, but cells recover afterwards; do not change medium during this time. Masimum density at 3-5x10^6 cells/mL. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 20% FBS; 10% (v/v) DMSO